Journal
YEAST
Volume 19, Issue 15, Pages 1295-1298Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/yea.909
Keywords
Saccharomyces cerevisiae; potassium analysis; rubidium analysis; HPLC
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Rubidium is widely used as a potassium analogue in transport studies in yeast and other organisms. As rubidium (potassium) uptake is modulated by the internal potassium concentration, it is often necessary to determine both Rb+ and K+ concentrations in the same cell extract. Current methods based on atomic absorption/emission spectroscopy require separate analysis for each cation. Alternatively, unsafe radioactive isotopes can be used. Here we report a convenient, non-radioactive, HPLC/conductivity-based method that allows a complete analysis of both cations with a single injection from a cell extract. The increase in Rb+ uptake during K+ starvation in yeast is easily demonstrated with this method. Copyright (C) 2002 John Wiley Sons, Ltd.
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