Journal
MARINE POLLUTION BULLETIN
Volume 44, Issue 11, Pages 1206-1218Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0025-326X(02)00177-7
Keywords
acropora cervicornis; Florida keys; dibrom; biomarkers; stressor specific response; stress gene expression
Ask authors/readers for more resources
We characterize two genes expressed in Acropora cervicornis upon exposure to 0.5 mug/l of dibrom, a pesticide used for mosquito control in the Florida Keys. Fragments of these genes were isolated, sequenced, and developed into chemiluminescent probes for Northern slot blots. Expression of target transcripts was detected in corals exposed to a variety of stressors including organophosphates, organochlorines, heavy metals, naphthalene, and temperature. Within the context of stressors examined, the D25 probe demonstrates toxicant and concentration specificity for organophosphates, whereas the D50 probe had broader specificity, detecting transcripts in corals exposed to dibrom, naphthalene, and temperature stress. After characterizing specificity in the lab, these probes were used on field samples taken from the Florida Keys. Both probes detected their targets in samples taken from the upper Florida Keys in August 2000. Preliminary search of sequence databases suggest similarity exists between D25 and a thioesterase. (C) 2002 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available