Journal
APPLIED OPTICS
Volume 41, Issue 31, Pages 6614-6620Publisher
OPTICAL SOC AMER
DOI: 10.1364/AO.41.006614
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We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 mum x 20 mum square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied. (C) 2002 Optical Society of America.
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