Journal
CHEMICAL & PHARMACEUTICAL BULLETIN
Volume 50, Issue 11, Pages 1467-1470Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.50.1467
Keywords
glycosphingolipid; glucocerebroside; sea cucumber; Holothuria pervicax; recycling HPLC
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Ten glucocerebrosides, HPC-3-A-HPC-3-J, have been isolated from their obtained parent glucocerebroside molecular species HPC-3, together with other glucocerebroside molecular species HPC-1 and HPC-2, from the less polar lipid fraction of a chloroform/methanol extract of the sea cucumber Holothuria pervicax. The structures of these glucocerebrosides have been determined on the basis of chemical and spectroscopic evidence. Reversed-phase HPLC, including a recycling system, was effective in isolating these glucocerebrosides, revealing a very close resemblance in structure, though the problem due to regio-isomers remains.
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