Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 50, Issue 23, Pages 6865-6870Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf025620t
Keywords
faba bean; legume phytase; lupine; myo-inositol phosphate isomers; phytate degradation
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Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytases purified from faba bean and lupine seeds, respectively, was established. The data demonstrate that the legume seed phytases under investigation dephosphorylate myo-inositol hexakisphosphate in a stereospecific way. The phytase from faba bean seeds and the phytase LP2 from lupine seeds degrade phytate by sequential removal of phosphate groups via D-Ins(1,2,3,5,6)P-5, D-Ins(1,2,5,6)P-4, D-Ins(1,2,6)P-3, and D-Ins(1,2)P-2 to finally Ins(2)P, whereas the phytases LP11 and LP12 from lupine seeds generate the final degradation product Ins(2)P via D-Ins(1,2,4,5,6)P-5, D-Ins(1,2,5,6)P-4, D-Ihs(1,2,6)P-3, and D-Ins(1,2)P-2.
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