Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 30, Issue 4, Pages 1403-1410Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0731-7085(02)00456-9
Keywords
vitamins B1,B6,B12; determination; multivitamin tablets; high performance liquid chromatography
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A simple, precise, rapid and selective HPLC-RP method has been developed for the simultaneous determination of thiamine hydrochloride (B-1) 150 mg, pyridoxine hydrochloride (B-6) 150 mg, and hydroxocobalamine chloride (1312) 0.150 mg in multivitamin tablets. The method uses a Hypersil-BDS C-18 reversed phase column and gradient elution. The aqueous mobile phase contained 0.015% triethylamine adjusted to pH 2.7 with 1 N sulfuric acid and acetonitrile. Separation and quantitation was achieved by changing the proportion of the system linearly with a time-schedule programme. Detection was carried out using a dual-beam UV detector set at 280, 350 nm. Good linearity was observed between the concentration of the analytes and peak area (r = 0.9999, 0.9998). Sample preparation was relatively simple whereas excipients present in the dosage forms did not interfere with the peaks of interest. Recovery of the compound from the B-1, B-6, B-12 was quantitative. (C) 2002 Elsevier Science B.V. All rights reserved.
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