Journal
NATURE
Volume 420, Issue 6911, Pages 85-89Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature01147
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- Fondazione Telethon Funding Source: Custom
- Telethon [485/B] Funding Source: Medline
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Membrane phosphoinositides control a variety of cellular processes through the recruitment and/or regulation of cytosolic proteins(1-4). One mechanism ensuring spatial specificity in phosphoinositide signalling is the targeting of enzymes that mediate their metabolism to specific subcellular sites. Phosphatidylinositol phosphate kinase type 1gamma (PtdInsPKIgamma) is a phosphatidylinositol-4-phosphate 5-kinase that is expressed at high levels in brain, and is concentrated at synapses(5,6). Here we show that the predominant brain splice variant of PtdInsPKIg (PtdInsPKIgamma-90) binds, by means of a short carboxy-terminal peptide, to the FERM domain of talin, and is strongly activated by this interaction. Talin, a principal component of focal adhesion plaques 7,is also present at synapses. PtdInsPKIgamma-90 is expressed in non-neuronal cells, albeit at much lower levels than in neurons, and is concentrated at focal adhesion plaques, where phosphatidylinositol-4,5-bisphosphate has an important regulatory role. Overexpression of PtdInsPKIgamma-90, or expression of its C-terminal domain, disrupts focal adhesion plaques, probably by local disruption of normal phosphoinositide balance. These findings define an interaction that has a regulatory role in cell adhesion and suggest new similarities between molecular interactions underlying synaptic junctions and general mechanisms of cell adhesion.
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