Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 780, Issue 1, Pages 111-117Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1570-0232(02)00441-5
Keywords
fish; florfenicol
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A simple method was developed for determining florfenicol concentration in a small volume (250 mul) of plasma from five phylogenetically diverse species of freshwater fish. Florfenicol was isolated from the plasma matrix through C-18 solid-phase extraction and quantified by reversed-phase high-performance liquid chromatography with UV detection. The accuracy (84-104%), precision (%RSDless than or equal to8), and sensitivity (quantitation limit <30 ng/ml) of the method indicate its usefulness for conducting pharmacokinetic studies on a variety of freshwater fish. Published by Elsevier Science B.V.
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