Journal
JOURNAL OF CELL BIOLOGY
Volume 159, Issue 4, Pages 625-635Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200208154
Keywords
TIR-FM; CD63; dextran; VAMP7; Synaptotagmin VII
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Funding
- NIGMS NIH HHS [R01 GM64625, R01 GM064625] Funding Source: Medline
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Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures. Using total internal reflection fluorescence microscopy, we observed that many organelles implicated in this process, including ER, post-Golgi vesicles, late endosomes, early endosomes, and lysosomes, were within 100 nm of the plasma membrane (in the evanescent field). However, an increase in cytosolic calcium led to exocytosis of only the lysosomes. The lysosomes that fused were predominantly predocked at the plasma membrane, indicating that calcium is primarily responsible for fusion and not recruitment of lysosomes to the cell surface.
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