Journal
JOURNAL OF CELL BIOLOGY
Volume 159, Issue 4, Pages 685-694Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200204102
Keywords
bladder; urothelium; tetraspanin; membrane; DNA mismatch repair enzyme
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Funding
- NIDDK NIH HHS [R01 DK039753, R01 DK057269, P01 DK052206, DK52206, DK57269, DK39753] Funding Source: Medline
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Urothelial plaques consist of four major uroplakins (Ia, Ib, II, and III) that form two-dimensional crystals covering the apical surface of urothelium, and provide unique opportunities for studying membrane protein assembly. Here, we describe a novel 35-kD urothelial plaque-associated glycoprotein that is closely related to uroplakin III: they have a similar overall type I transmembrane topology; their amino acid sequences are 34% identical; they share an extracellular juxtamembrane stretch of 19 amino acids; their exit from the ER requires their forming a heterodimer with uroplakin Ib, but not with any other uroplakins; and UPIII-knockout leads to p35 up-regulation, possibly as a compensatory mechanism. Interestingly, p35 contains a stretch of 80 amino acid residues homologous to a hypothetical human DNA mismatch repair enzyme-related protein. Human p35 gene is mapped to chromosome 7q11.23 near the telomeric duplicated region of Williams-Beuren syndrome, a developmental disorder affecting multiple organs including the urinary tract. These results indicate that p35 (uroplakin IIIb) is a urothelial differentiation product structurally and functionally related to uroplakin 111, and that p35-UPlb interaction in the ER is an important early step in urothelial plaque assembly.
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