Analysis of tropane and related alkaloids

The current methods for tropane alkaloid chromatographic separation and determination are summarised. The alkaloids included are: the medicinally applied tropic acid esters hyoscyamine and scopolamine and their derivatives, cocaine and derivatives, the metabolites and degradation products of these compounds occurring in plant material, calystegines as nortropane alkaloids, anatoxins as homonortropane alkaloids, pelletierines and pseudopelletierines as alkaloids with isomeric structures. Developments in GC, HPLC, CE and TLC are presented and the advantages of each method for plant analysis are discussed. A summary for each chromatographic method lists the instrumentation and parameters applied for tropane alkaloids. (C) 2002 Elsevier Science B.V. All rights reserved.