Antioxidant effect of hydroxytyrosol (DPE) and Mn2+ in liver of cadmium-intoxicated rats

Liver TBARS formation in cadmium-intoxicated rats was completely reduced by administering a low amount of MnCl2 (2 mg/kg b.w.) I h before intoxication. A similar antioxidant effect was first shown by hydroxytyrosol (2-(3,4-dihydroxyphenyl)ethanol, (I)PE), a phenolic compound present in olive oil, given twice to rats (9 mg/kg b.w.) after cadmium administration. The antioxidant properties shown in vivo by both Mn2+ and DPE were also active in vitro when rat liver microsomes were subjected to lipid peroxidation by cadmium or other prooxidant systems. The increase in liver glutathione concentrations occurring in cadmium-intoxicated rats, was also found, for the first time, 24 h after MnCl2 administration. Unlike cadmium intoxication, which caused a higher formation of both glutathione and TBARS, Mn2+ induced glutathione synthesis without any TBARS formation. The same situation was also observed when cadmium plus Mn2+ or cadmium plus DPE was given to rats. Our data show that: (a) both DPE and low Mn2+ concentrations may have an antioxidant effect in the livers of cadmium-intoxicated rats and (b) Mn2+, like cadmium, induces liver glutathione synthesis and this effect is probably independent of TBARS formation. (C) 2002 Elsevier Science Inc. All rights reserved.