Journal
GENES & DEVELOPMENT
Volume 16, Issue 23, Pages 2985-2990Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1040502
Keywords
zinc finger; mitosis; phosphorylation; Ikaros; cell cycle
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Funding
- NCI NIH HHS [CA82430, CA009120, T32 CA009120] Funding Source: Medline
- NICHD NIH HHS [HD07512, T32 HD007512] Funding Source: Medline
- NIDDK NIH HHS [R01 DK043726, DK43726] Funding Source: Medline
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Many nuclear proteins are inactivated during mitotic entry, presumably as a prerequisite to chromatin condensation and cell division. C2H2 zinc fingers define the largest transcription factor family in the human proteome. The linker separating finger motifs is highly conserved and resembles TGEKP in more than 5000 occurrences. However, the reason for this conservation is not fully understood. We demonstrate that all three linkers in the DNA-binding domain of Ikaros are phosphorylated during mitosis. Phosphomimetic substitutions abolished DNA-binding and pericentromeric localization. A linker within Sp1 was also phosphorylated, suggesting that linker phosphorylation provides a global mechanism for inactivation of the C2H2 family.
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