Journal
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
Volume 34, Issue 12, Pages 1569-1576Publisher
ELSEVIER SCI LTD
DOI: 10.1006/jmcc.2002.2080
Keywords
angiotensin 1-7; angiotensin 1-9; B-2 receptor; ACE
Categories
Funding
- NHLBI NIH HHS [HL68580, HL36473] Funding Source: Medline
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Some beneficial effects of ACE inhibitors are attributed to potentiation of bradykinin's actions exerted through its B-2 receptor. We investigated them on cultured cells transfected or constitutively expressing both ACE and B-2 receptor. The potentiation of bradykinin was indirect and attributed to a crosstalk induced between enzyme and receptor via ACE, a heterodimer formation. While looking for endogenous activators, we investigated the split products of angiotensin I (Ang) Ang 1-9 and 1-7, peptides released by enzymes of human atria and ventricle. Ang 1-9 was liberated by a cathepsin A-type enzyme, Ang 1-7 by a different metallopeptidase-protease. Cathepsin A's presence in heart tissue was shown by deamidating enkephalinamide substrate, by immunoprecipitation and by inummohistochemistry. In immunohistochemistry, cathepsin A was detected in myocytes of atrial tissue. Ang 1-9 and Ang 1-7 potentiated the effect of an ACE-resistant bradykinin analogue on the B-2 receptor in transfected cells expressing human ACE and B-2, and in human endothelial cells. Ang 1-9 and 1-7 augmented arachidonic acid and NO release by bradykinin. NO liberation by bradykinin from endothelial cells was potentiated at 10 nmol/L concentration by Ang 1-9 and Ang 1-7; at higher concentrations, Ang 1-9 was significantly more active. Both peptides had little activity in absence of bradykinin or ACE. Ang 1-9 and 1-7 potentiated bradykinin action on its B2 receptor at much lower concentrations than their IC50 values with ACE. They probably induce conformational changes in the ACE/B-2 receptor complex via interaction with ACE. (C) 2002 Elsevier Science Ltd. All rights reserved.
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