Journal
MEDICAL AND VETERINARY ENTOMOLOGY
Volume 16, Issue 4, Pages 461-464Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2915.2002.00393.x
Keywords
Anopheles gambiae complex; malaria vectors; molecular markers; mosquito identification; PCR-RFLP; sibling species; Africa
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For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
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