Journal
EUKARYOTIC CELL
Volume 1, Issue 6, Pages 1041-1044Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.1.6.1041-1044.2002
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Funding
- NHLBI NIH HHS [HL67401, R01 HL067401] Funding Source: Medline
- NIAID NIH HHS [AI47718, AI46768, R01 AI047718] Funding Source: Medline
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Yeasts respond to treatment with azoles and other sterol biosynthesis inhibitors by upregulating the expression of the ERG genes responsible for ergosterol production. Previous studies on Saccharomyces cerevisiae implicated the ROX1 repressor in ERG regulation. We report that ROX1 deletion resulted in 2.5- to 16-fold-lower susceptibilities to azoles and terbinafine. In untreated cultures, ERG11 was maximally expressed in mid-log phase and expression decreased in late log phase, while the inverse was observed for ROX1. In azole-treated cultures, ERG11 upregulation was preceded by a decrease in ROX1 RNA. These inverse correlations suggest that transcriptional regulation of ROX1 is an important determinant of ERG expression and hence of azole and terbinafine susceptibilities.
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