Journal
CHINESE SCIENCE BULLETIN
Volume 47, Issue 24, Pages 2095-2099Publisher
SCIENCE CHINA PRESS
DOI: 10.1360/02tb9455
Keywords
M. gryphiswaldense; submerged culture; nitrogen fixation; regulation of nitrogenase activity
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A submerged culture technique for Magneto-spirillum gryphiswaldense under the nitrogen-fixing condition (microaerobic and N-limited) was set up. In N-limited medium with Na-lactate as a sole carbon source, the optical density (A(600 nm))and activity of nitrogen fixation of cells were 1.3 and 217 nmol of ethylene produced per hour per A(600 nm) respectively within 21 h by three times of feeds. The pH and temperature were controlled at 7.2 and 30degreesC respectively, and the oxygen concentration was controlled by sparging with N-2 containing 0.4%-0.8% of O-2. The activity of nitrogen fixation of cells was obviously inhibited by oxygen and ammonium. It indicated that the posttranslational regulation of nitrogenase existed in M. gryphiswaldense.
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