Journal
CURRENT GENETICS
Volume 42, Issue 3, Pages 147-152Publisher
SPRINGER-VERLAG
DOI: 10.1007/s00294-002-0342-7
Keywords
yeast; tRNA regulation; translational fidelity; antisuppression
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Maf1p is a negative effector of RNA polymerase III in yeast. The maf1-Delta mutation caused an increase in the level of cellular tRNAs, but a decrease of translational readthrough at nonsense codons. Using the lacZ-luc dual gene reporter system, we detected an almost twofold diminution of UAA and UAG readthrough in maf1-Delta compared with the parental strain. The maf1-Delta mutation did not affect the rate of protein biosynthesis and growth at standard conditions, but resulted in temperature-sensitive growth on nonfermentable carbon sources. We examined the correlation of the temperature sensitive and antisuppression phenotypes of maf1-Delta using a colour phenotype assay in the ade2-1 SUP11 strain. Antisuppression, but not the temperature-sensitive growth defect, was compensated either by increased dosage of SUP11 or by [PS1(+)], the prion form of the translation termination factor Sup35p. Summarizing, the elevated tRNA levels in maf1-Delta increase translational fidelity and, independently, affect growth under special conditions.
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