Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 133, Issue 4, Pages 581-595Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1096-4959(02)00166-5
Keywords
cytosine methylation; southern analysis; fish; inter-species hybrid; backcross genetics
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Funding
- NCI NIH HHS [CA75137] Funding Source: Medline
- NCRR NIH HHS [RR17072, RR12253] Funding Source: Medline
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Cytosine methylation appears to be established as an important DNA base modification involved in regulation of gene expression but is poorly understood from an evolutionary viewpoint. Xiphophorus progeny from inter-species crosses and backcrosses that are utilized in contemporary tumor induction studies were analyzed for cytosine methylation pattern inheritance using Southern blot analyses. Methylation patterns at CCGG sequences of 411 independent chromosomes in three distinct inter-species crosses were analyzed. In every case the non-recurrent parental methylation pattern remained unaltered for each of the genes studied, once introduced into the recurrent parental genetic background. Through F, inter-species hybridization and succeeding meiosises leading to first generation (BC1) and second generation (BC2) backcross hybrid progeny, we demonstrate that parental species methylation patterns are stable. (C) 2002 Elsevier Science Inc. All rights reserved.
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