Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 6, Issue 6, Pages 823-828Publisher
CURRENT BIOLOGY LTD
DOI: 10.1016/S1367-5931(02)00394-0
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- NIGMS NIH HHS [GM49243] Funding Source: Medline
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Less than a decade old, single-molecule fluorescence of nucleic acids has rapidly become an important tool in the arsenal of biological probes. A variety of novel approaches to investigate conformational dynamics, catalytic mechanisms, folding pathways and protein-nucleic-acid interactions have recently been devised for nucleic acids using this technique. Combined with biomechanical tools and ensemble measurements, single-molecule fluorescence methods extend our ability to observe and understand biomolecules and complex biological processes.
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