Journal
MOLECULAR ECOLOGY NOTES
Volume 2, Issue 4, Pages 456-458Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8286.2002.00276.x
Keywords
enrichment; microsatellites; paternity analysis; Poeciliidae; population
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We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33-48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.
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