Journal
TOXICON
Volume 40, Issue 12, Pages 1723-1726Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0041-0101(02)00202-7
Keywords
Staphylococcal enterotoxins; detection; ELISA
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We present here simple, sensitive and accurate colorimetric capture ELISAs for staphylococcal enterotoxins A and B. Standard curves were linear over the range 0.5-1 ng/mL, and toxins could be accurately measured at 0.5 ng/mL in assay buffer or 0.1 ng/mL in human urine. Cross-reactivity between serotypes was negligible. Detection in serum was complicated by the presence of specific antibodies to SE's in most normal sera. These assays offer a viable, cost-effective method for analysis of these ubiquitous toxins. Further, their sensitivity in undiluted urine makes them ideal candidates for evaluating human exposure. Published by Elsevier Science Ltd.
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