Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 74, Issue 4, Pages 649-675Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/S0001-37652002000400007
Keywords
trypanosomatids; cell surface charge; electrophoretic mobility; cytochemistry
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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.
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