Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 66, Issue 12, Pages 2715-2718Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.66.2715
Keywords
sericin; Bombyx mori; SDS-PAGE; cocoon; middle silk gland
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To characterize the sericin components of the cocoon of silkworm Bombyx mori, fresh cocoon shells were dissolved in saturated aqueous lithium thiocyanate containing 2-mercaptoethanol, and fractionated by ethanol precipitation. Cocoon sericin was found to mainly consist of three polypeptides having molecular masses of the 400, 250, and 150 kDa estimated by SDS-PAGE, which corresponds to the sericin present in the middle, anterior, and posterior part of the middle silk gland. The amino acid compositions of the 400 and 150 kDa components were similar to each other, but that of the 250 kDa component was different. This suggests differences in the coding gene and properties of the 250 kDa sericin from the other two.
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