Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 14, Issue 6, Pages 497-500Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1022314815045
Keywords
green alga; Haematococcus pluvialis; lacZ; micro-particle bombardment; SV40 promoter
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This paper reports for the first time the transient expression of a reporter gene, LacZ, in the unicellular green alga Haematococcus pluvialis. By employing the micro-particle bombardment method, motile cells in the exponential phase showed transient expression of lacZ. This was detected in bombarded motile cells under the rupture-disc pressures of 3103 KPa and 4137 KPa. Transient expression of LacZ gene could not be observed in non-motile cells of this alga under the same transformation condition. No LacZ background was found in either the motile cells or the non-motile cells. The study suggests a promising potential of the SV40 promoter and the lacZ reporter gene in genetic engineering of unicellular green algae.
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