Journal
IMMUNITY
Volume 17, Issue 6, Pages 795-808Publisher
CELL PRESS
DOI: 10.1016/S1074-7613(02)00482-X
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Funding
- NIAID NIH HHS [R01 AI47864] Funding Source: Medline
- NIDDK NIH HHS [R21 DK61333] Funding Source: Medline
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Long-term display of exogenous proteins on the cell surface may have important research and therapeutic implications. We report a novel method for the cell-surface display of proteins that involves generation of a chimeric protein with core streptavidin, biotinylation of cells, and decoration with the protein. A chimeric protein with the extracellular portions of FasL (SA-FasL) was efficiently displayed on the cell surface within 2 hr without detectable cellular toxicity. Biotin and SA-FasL persisted on the cell surface for weeks in vitro and in vivo. Immunomodulation with SA-FasL-decorated splenocytes effectively blocked alloreactive responses in naive and presensitized rodents and prevented the rejection of allogeneic pancreatic islets. This approach may serve as an alternative to gene transfer-based expression with broad research and therapeutic applications.
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