Journal
FEBS LETTERS
Volume 532, Issue 1-2, Pages 31-35Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)03620-7
Keywords
extracellular matrix; collagenase-3; transforming growth factor-beta 1 signaling; breast cancer metastasis
Funding
- NIDDK NIH HHS [DK-47420, R01 DK047420] Funding Source: Medline
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Transforming growth factor (TGF)-beta1, a crucial molecule in metastatic bone cancer, stimulates collagenase-3 expression in the human breast cancer cell line, MDA-MB231. Cycloheximide inhibited this stimulation, indicating that de novo protein synthesis was essential for this response. We examined whether mitogen-activated protein kinase (MAPK) and/or Smad pathways are involved in TGF-beta1-stimulated collagenase-3 expression in MDA-MB231 cells. Biochemical blockade of extracellular regulated kinase-1/2 and p38 MAPK pathways partially abolished TGF-beta1-stimulated collagenase-3 mRNA expression; whereas overexpression of a dominant negative form of Smad3 completely blocked the TGF-beta1-response. These data indicate that TGF-beta1-induced MAPK and Smad pathways are involved in TGF-beta1-stimulated collagenase-3 expression in MDA-MB231 cells. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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