Journal
JOURNAL OF CELL BIOLOGY
Volume 159, Issue 5, Pages 855-866Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200208112
Keywords
membrane fusion; p97; VCIP135; Golgi; ER
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Funding
- Wellcome Trust Funding Source: Medline
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NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPS/SNARES complexes, separating the v- and t-SNARES, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP] [p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP735 and p47 are shown to function in Golgi and ER assembly.
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