Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume 1579, Issue 2-3, Pages 133-141Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-4781(02)00532-8
Keywords
VDAC; rice; promoter; mitochondrion; channel
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The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. The putative promoter of the rice vdac isoform2 (osvdac2) was isolated by screening a rice genomic library. Computer-based analyses predicted a TATA box, a putative transcription start and several transcription factor-binding sites including pollen specific elements. The promoter region was fused to the gus reporter gene and introduced into rice by Agrobacterium-mediated transformation. Histochemical and cell-type localizations indicated an overall expression of this promoter with a strong expression in actively growing lateral roots and in the pollen grains. Quantitative experiments showed that the osvdac2 promoter has a strong specific activity in both root and shoot. Thus, the osvdac2 promoter could be a good alternative to viral promoters (e.g. CaMV 35S) to overexpress genes in transgenic Poaceae. (C) 2002 Elsevier Science B.V All rights reserved.
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