Journal
JOURNAL OF IMMUNOLOGY
Volume 169, Issue 12, Pages 6985-6991Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.169.12.6985
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Funding
- NHLBI NIH HHS [HL70876] Funding Source: Medline
- NIAID NIH HHS [AI48167] Funding Source: Medline
- PHS HHS [02878, 12878, 13948, 016692] Funding Source: Medline
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In a search for direct evidence leading to the biological relevance of airway secretions in innate host defense, we characterized the antibacterial function of cationic polypeptides within minimally manipulated nasal fluid. In this study, we show that cationic antimicrobial polypeptides are responsible for most of the bactericidal activity of whole nasal fluid. The removal of cationic polypeptides using a cation-exchange resin ablated the activity of nasal fluid against Escherichia coli, Listeria monocytogenes, and Pseudomonas aeruginosa. By using a novel proteomic approach, we identified a dozen cationic peptides and proteins within nasal fluid, all of which either are known antimicrobial polypeptides or have other proposed roles in host defense. Of the three most abundant cationic polypeptides in nasal fluid, lysozyme was more effective than either lactoferrin or secretory leukoprotease inhibitor in restoring the antibacterial activity of the cationic polypeptide-depleted fluid against a mucoid cystic fibrosis isolate of P. aeruginosa.
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