Journal
JOURNAL OF IMMUNOLOGY
Volume 169, Issue 12, Pages 6659-6663Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.169.12.6659
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Funding
- NIAID NIH HHS [P01 AI35296, T32 AI07313] Funding Source: Medline
- NIGMS NIH HHS [R01 GM54706] Funding Source: Medline
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The role of B7/CD28 signals in Ag-induced cell cycle progression of CD4(+) T cells was examined using the technique of CFSE dye dilution and flow cytometry. In wild-type T cells, proliferation was directly related to the concentration of Ag available to the APC. Consistent with this, the rate of G(0)-->G(1) cell cycle progression varied with the concentration of Ag. However, cell division by T cell blasts occurred at a constant rate, independent of Ag concentration. G(0)-->G(1) phase progression by CD28-deficient CD4(+) T cells or wild-type T cells cultured in the presence of neutralizing anti-B7 mAbs was slowed, confirming that a synergy does exist between TCR and CD28 signaling in the initial activation of the T cells. However, unlike the TCR, the strength of CD28 stimulation was also shown to play a unique role in controlling the rate of cell division by T cell blasts.
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