Journal
SCIENCE
Volume 298, Issue 5602, Pages 2353-2358Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1078124
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Funding
- NHLBI NIH HHS [HL20948] Funding Source: Medline
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The low-density lipoprotein receptor mediates cholesterol homeostasis through endocytosis of lipoproteins. It discharges its ligand in the endosome at pH<6. In the crystal structure at pH=5.3, the ligand-binding domain (modules R2 to R7) folds back as an arc over the epidermal growth factor precursor homology domain (the modules A, B, beta propeller, and C). The modules R4 and R5, which are critical for lipoprotein binding, associate with the beta propeller via their calcium-binding loop. We propose a mechanism for lipoprotein release in the endosome whereby the beta propeller functions as an alternate substrate for the ligand-binding domain, binding in a calcium-dependent way and promoting lipoprotein release.
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