Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 299, Issue 5, Pages 872-879Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0006-291X(02)02753-5
Keywords
transcription factor; ERR3; NR3B3; nuclear localization; EGFP; transactivation; pull down
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The mouse nuclear receptor ERRgamma (estrogen receptor-related receptor 7) is highly expressed in heart, skeletal muscle, kidney, and brain, as well as in the developing nervous system. We found that the expression of the coactivators PGC-1 (PGC-1alpha) and PERC (PGC-1beta) in mammalian cells augmented potently the transcriptional activation by ERRgamma. The constitutive activation function 2 (AF-2) of the orphan receptor was important for the synergistic enhancement. Functional receptor truncation analysis revealed an additional amino-terminal activation function, specific for the ERRgamma2 isoform and PGC-1. In vitro experiments showed a direct interaction of ERRgamma with both coactivators. Our findings suggest distinct regulatory functions for PGG-1 and PERC as tissue-specific coactivators for ERRgamma. (C) 2002 Elsevier Science (USA). All rights reserved.
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