Journal
JOURNAL OF APICULTURAL RESEARCH
Volume 54, Issue 1, Pages 36-39Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00218839.2015.1024029
Keywords
TLC; allyl 3-prenylcinnamate; authentication; Brazilian green propolis
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Funding
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
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The detection of marker compounds is a necessary step for chemical characterization and standardization of propolis. A thin-layer chromatography procedure is described for the detection of allyl 3-prenylcinnamate, a volatile marker of Brazilian green propolis. The powdered sample is heated inside a petri dish and the material condensed on the dish cap is chromatographed on silica gel plates. The visualization may be done by spraying the plates with a solution of sodium fluorescein or a solution of sulphuric acid vanillin reagent.
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