Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 982, Issue 2, Pages 237-244Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)01650-3
Keywords
tea; food analysis; amino acids; carbohydrates
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Determination of amino acids and sugars in green tea by anion-exchange chromatography with integrated pulsed amperometric detection was developed. Amino acids and sugars were separated on an anion-exchange column at a flow-rate of 0.25 ml/min by using ternary gradient elution consisting of deionized water, 0.25 M sodium hydroxide, and 1.0 M sodium acetate. Under optimized conditions, theanine was separated from glutamine and three sugars (glucose, fructose, and sucrose) were eluted earlier than the neutral amino acids to avoid their interference with each other. RSDs of the peak area of analytes were lower than 4.6%. Detection limits for the analytes ranged from 0.12 to 4.9 pmol. The linearities for all analytes were two or three orders of magnitude, with the correlation coefficients greater than 0.99. This method was applied to determination of amino acids and sugars in green tea with satisfactory results. (C) 2002 Elsevier Science B.V. All rights reserved.
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