Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1619, Issue 1, Pages 70-76Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4165(02)00444-0
Keywords
oxalate; phosphatidylethanolamine (PE); aluminum; isocitrate lyase
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C-13 NMR studies with aluminum (Al)-stressed Pseudomonas fluorescens revealed that the trivalent metal was secreted in association with oxalate and phosphatidylethanolamine (PE). These moieties were observed in the insoluble pellet obtained upon incubation of these resting cells in the presence of either Al-citrate or citrate. This extrusion process was concomitant with the utilization of either of these tricarboxylic acids as a substrate. While only minimal amounts of At were secreted in the presence of such carbon source as glucose, succinate or oxaloacetate, oxalate did pen-nit the efflux of Al. Neither alpha-ketoglutarate nor ethylenediaminetetraacetic acid (EDTA) was effective in dislocating Al from the cells. The elimination of Al from the cells did not appear to be affected by p-dinitrophenol (DNP) or dicyclohexylcarbodiimide (DCCD) or azide, but was sensitive to temperature, pH and cerulenin, an inhibitor of lipid synthesis. Thus, it appears that R fluorescens detoxifies At via its extrusion in association with oxalate and PE in a process that apparently does not necessitate the direct utilization of energy. (C) 2002 Elsevier Science B.V All rights reserved.
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