Journal
CANCER LETTERS
Volume 189, Issue 1, Pages 69-75Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3835(02)00503-7
Keywords
shock tube; saporin; cancer; ribosome-inactivating protein; cytoplasmic delivery
Categories
Funding
- NCI NIH HHS [R01 CA/AI838801, R01 CA083882] Funding Source: Medline
- NIAID NIH HHS [R01 AI050875] Funding Source: Medline
Ask authors/readers for more resources
We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10(-9) M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available