Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 272, Issue 1-2, Pages 177-187Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(02)00499-4
Keywords
tetrameric antigen; antigen-specific B cells; flow cytometry; flumoral immune response
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The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses.' We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labeled antigen is made tetramdric by interaction with streptavidin. The enhanced avidity of this antigenic compound for the B cell membrane permits the visualization, characterization and isolation of antigen-specific B cells. (C) 2002 Elsevier Science B.V All rights reserved.
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