Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 300, Issue 3, Pages 637-644Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0006-291X(02)02907-8
Keywords
Apg8/Aut7; LC3; GABARAP; GATE-16; ubiquitin-like modification; Apg7; Apg3; autophagy; lipidation
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GATE-16, GABARAR and LC3 are three mammalian counterparts of yeast Apg8p/Aut7p. Here. we show that GATE-16 and GABARAP are authentic modifiers, as is the case of LC3 modification. The C-terminal Phet(117) of proGATE-16 and the C-terminal Leu(117) of proGABARAP are post-translationally cleaved to expose an essential Gly(116) within GATE-16 and GABARAP, with the products designated GATE-16-I and GABARAP-I. respectively. The Gly(116) within GATE-16 and GABARAP are essential for further formation of the intermediates between them and Apg7p(C572S) and Apg3p(C264S). When Apg7p and Apg3p are expressed. GATE-16-I and GABARAP-I are modified to a secondary ubiquitin-like modified form, GATE-16-II and GABARAP-II, respectively. GATE-16-I and GABARAP-I, but not LC3-I, localize to membrane compartments before their modification. These results indicate that GATE-16 and GABARAP are authentic modifiers, but that they have different biochemical characteristics from those of LC3. (C) 2002 Elsevier Science (USA). All rights reserved.
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