Journal
JOURNAL OF CONTROLLED RELEASE
Volume 86, Issue 2-3, Pages 349-359Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-3659(02)00365-6
Keywords
chitosan; galactose; poly(vinyl pyrrolidone); DNA carrier; hepatocyte
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Galactosylated chitosan was conjugated with poly(vinyl pyrrolidone) (PVP) as a hydrophilic group. The complex formation of GC-graft-PVP (GCPVP)/DNA complexes was confirmed by agarose gel electrophoresis. The morphology of the complex observed by atomic force microscopy had a compact and spherical shape, around 40 nm particle sizes at a charge ratio of 3. The binding strength of GCPVP 10K/DNA complex measured by ethidium bromide binding assay was superior to that of the GCPVP 50K/DNA one, probably attributable to its higher flexibility due to the smaller size, whereas the DNase I protection of GCPVP 10K/DNA complex was inferior to that of the GCPVP 50K/DNA one. This indicated that effective complex formation required both higher binding strength and minimal molecular weight of polycation enough to induce the condensation of DNA. The DNA-binding property of GCPVP mainly depended on the molecular weight of chitosan and composition of PVP. (C) 2002 Elsevier Science B.V. All rights reserved.
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