Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume 1625, Issue 2, Pages 221-226Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-4781(02)00625-5
Keywords
Ilyobacter tartaricus; propionigenium modestum; atp-operon; inverse PCR; F1Fo ATP synthase; subunit a
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The atp operon of Ilyobacter tartaricus, strain DSM 2382, was completely sequenced using conventional and inverse polymerase chain reaction (i-PCR) techniques. It contains nine open reading frames that were attributed to eight structural genes of the F1Fo ATP synthase and the atpI gene, which is not part of the enzyme complex. The initiation codons of all atp genes, except that of atpB coding for the a subunit, were identified by the corresponding N-terminal amino acid sequence. The hydrophobic a subunit was identified by MALDI mass spectrometry. The atp genes of I. tartaricus are arranged in one operon with the sequence atpIBEFHAGDC comprising 6992 base pairs with a GC content of 38.1%. The F1Fo ATP synthase of I. tartaricus has a calculated molecular mass of 510 kDa and includes 4810 amino acids. The gene sequences and products reveal significant identities to atp genes of other Na+-translocating F1Fo ATP synthases, especially in the F-o subunits a and c which are directly involved in ion translocation. (C) 2002 Elsevier Science B.V. All rights reserved.
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