Journal
PHYTOCHEMISTRY
Volume 62, Issue 3, Pages 371-376Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(02)00556-3
Keywords
Zea mays; Triticum aestivum; DIMBOA biosynthesis; 2-oxoglutarate dependent oxygenase; gene cluster
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Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize. (C) 2003 Elsevier Science Ltd. All rights reserved.
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