Journal
FOOD CHEMISTRY
Volume 80, Issue 2, Pages 275-282Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0308-8146(02)00382-5
Keywords
Melissa officinalis l.; antioxidants; semi-batch extraction; phase equilibrium
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In the paper, the extraction of antioxidants from Balm (Melissa officinalis L.) leaves with ethanol is presented. Effects of particle size, amount of solvent and temperature on the extraction rates and concentrations of antioxidants in the extracts were studied and kinetics was determined. Individual antioxidants (carnosic, ursolic and oleanolic acids) were. identified by high performance liquid chromatography. Results showed that the intraparticle diffusion was the rate-governing step of the extraction process. The extractions all proceeded in three stages: an initial washing stage, a fast stage and a slower stage. Experimental extraction curves were analysed with a mathematical model derived from Fick's second law, and diffusion coefficients of the antioxidants within the particles under different operating conditions in ethanol were determined. (C) 2002 Elsevier Science Ltd. All rights reserved.
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