Yatakemycin, a novel antifungal antibiotic produced by Streptomyces sp TP-A0356

In the screening of novel antifungal compounds, yatakemycin was found in the culture broth of Streptomyces sp. TP-A0356. Yatakemycin was obtained by solvent extraction of the fermentation broth and chromatographic purification using ODS column and preparative HPLC. The structure of yatakemycin was elucidated by NMR and CID-MS/MS experiments as a novel antibiotic belonging to a family of CC-1065 and duocarmycins known to be DNA. alkylating agents. Yatakemycin inhibited the growth of pathogenic fungi; such as Aspergillus fumigatus and Candida albicans with the MIC values of 0.01 similar to 0.03 mug/ml, more potent than amphotericin B (MIC: 0.1 similar to 0.5 mug/ml) or itracohazole (MIC: 0.03 similar to 0.2 mug/ml). It also showed potent cytotoxicity against cancer cell lines with the IC50 of 0.01similar to0.3 mug/ml.