Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 4, Pages 311-314Publisher
SPRINGER
DOI: 10.1023/A:1022349401575
Keywords
glycerol; metabolite; osmotolerant yeast; O-2 supply; sulphite
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Candida glycerinogenes is an aerobe which does not depend on sulphite for production of glycerol. With a sufficient O-2 supply, up to 130 g glycerol l(-1) was produced with 2.6 g acetic acid l(-1) as by-product. However, with an insufficient O-2 supply - with higher volumes of medium or at higher corn steep liquid concentrations - the glycerol concentration was lower because the by-products, ethanol, pyruvate and lactic acid, were produced in greater amounts, up to 45 g l(-1), 4.3 g l(-1), 1.6 g l(-1), respectively, whereas, less acetic acid (0.6 g l(-1)) was produced. In addition, ethanol decreased to 0.4 g l(-1) and the glycerol yield improved from 34 to 50% (w/w) by adding 50 g sulphite l(-1), nevertheless, acetic acid increased to 7.8 g l(-1).
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