Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 70, Issue 12, Pages 3222-3229Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkv262
Keywords
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Funding
- Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III [PI11-02046, PI10/00056]
- Consejeria de Innovacion Ciencia y Empresa, Junta de Andalucia, Spain [P11-CTS-7730]
- Plan Nacional de I+D+i
- Instituto de Salud Carlos III, Subdireccion General de Redes y Centros de Investigacion Cooperativa, Ministerio de Economia y Competitividad
- Spanish Network for Research in Infectious Diseases [REIPI RD12/0015]
- European Development Regional Fund
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Objectives: The objective of this study was to analyse whether there is an association between reduced susceptibility to biocides in Acinetobacter baumannii and (i) antimicrobial resistance (co-resistance), (ii) prevalent (epidemic) clones, (iii) changes in the fitness or (iv) expression of genes related to efflux pumps and porins. Methods: Susceptibility to biocides and antimicrobials was determined in 49 clonally unrelated isolates of A. baumannii. Biological cost, in terms of mean generation time, was determined by spectrophotometry. Quantitative real-time RT-PCR was used to determine the relative expression of genes encoding several efflux pumps and porins. Results: Reduced susceptibility to chlorhexidine digluconate, benzalkonium chloride and Irgasan (R) was associated with resistance to aminoglycosides, tetracycline and ciprofloxacin (P<0.05). The MICs of carbapenems, aminoglycosides, doxycycline and ciprofloxacin for isolate Ab70 (epidemic clone) exposed to these biocides increased by >= 2 dilutions. Reduced susceptibility to Orsan (R) was more frequent among prevalent clones than non-prevalent clones (P < 0.05). Mean generation times for Ab70 before and after exposure to benzalkonium chloride were 57.8 and 78.1 min, respectively (P = 0.02). Relative expression of abeS and adeB was increased in Ab46 and Ab70 after exposure to chlorhexidine digluconate, but was decreased for ompA and carO after exposure to Irgasan (R). Conclusions: Reduced susceptibility to biocides is associated with co-resistance to carbapenems, aminoglycosides, tetracycline and ciprofloxacin. Reduced susceptibility to Orsan (R) may be a marker of prevalent clones. Acquisition of reduced susceptibility to benzalkonium chloride has a biological cost. Exposure to biocides affects the relative expression of genes related to some efflux pump genes (increased expression) or porins (reduced expression).
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