Journal
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
Volume 27, Issue 9, Pages 1355-1359Publisher
ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
DOI: 10.5713/ajas.2014.14046
Keywords
Goat; Vascular Endothelial Growth Factor (VEGF); Recombinant Protein; Hair Growth
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Funding
- Natural Sciences Foundation of China [31160469, 31360561]
- Natural Sciences Foundation of Inner Mongolia, China [2011MS0521]
- graduate student research project of Inner Mongolia University
- Major Projects for New Varieties of Genetically Modified Organisms [2013ZX08008-002]
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To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, fall-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6xhis-gVEGF164 protein was induced by 0.5 mM isopropyl thio-P-D-galactoside at 32 degrees C. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
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