Journal
PLANT SCIENCE
Volume 164, Issue 2, Pages 175-182Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-9452(02)00380-1
Keywords
chrysanthemum; dwarfing; GAI; gibberellin response; stem elongation
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000583] Funding Source: researchfish
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Several transgenic chrysanthemum (Chrysanthemum morifolium Ramat.) lines have been produced that express the Arabidopsis thaliana gai (gibberellic acid insensitive) gene under its own promoter. These transformants exhibit a range of dwarf phenotypes, the extent of dwarfing being related to the reduction in the response to gibberellin in each transgenic line. Physiological measurements of growth, chlorophyll content and flowering time demonstrate that the extent of the transgene effects correlate with the level of transgene expression. Production of many ornamental crops relies heavily on the use of expensive and harmful agrochemicals in order to produce a blemish free and uniform product. We demonstrate the feasibility of producing a dwarf (pot) chrysanthemum without the need for growth retardant chemicals through heterologous expression of the mutant Arabidopsis gai gene driven from its own promoter. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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