Journal
STRUCTURE
Volume 11, Issue 2, Pages 217-224Publisher
CELL PRESS
DOI: 10.1016/S0969-2126(03)00006-6
Keywords
radiation damage-induced phasing; trypsin; RNA; debromination; radiation damage; synchrotron radiation
Funding
- NIGMS NIH HHS [GM 56969, GM 61010] Funding Source: Medline
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The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, byway of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
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