Journal
RESEARCH IN VETERINARY SCIENCE
Volume 74, Issue 1, Pages 37-45Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0034-5288(02)00151-0
Keywords
atopy; atopic dermatitis; canine; dog; enzyme-linked immunosorbent assay (ELISA) immunoglobulin E (IgE); intradermal test; Prausnitz-Kustner test
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Twenty-eight atopic dogs, 22 pruritic, non-atopic dogs and 10 healthy dogs were ELISA tested. For calculations of diagnostic specificity and sensitivity, positive ELISA test results in non-atopic dogs were considered false positive results. The absence of any positive results in the atopic dogs was considered false negative results. The atopic dogs were tested both with ELISA and an intradermal test, utilising allergen extracts from the same manufacturer, to determine the frequency of positive allergen reactions in the ELISA test compared with the intradermal test. The Prausnitz-Kustner test was performed to evaluate the significance of a positive ELISA test result. Based on cross-tabulations with clinically defined atopic dermatitis, the ELISA test showed a sensitivity of 53.6% and a specificity of 84.4%. The correlation between the ELISA and the intradermal test was poor. Positive Prausnitz-Kustner tests were not obtained using sera from dogs that were intradermal test negative for the tested allergens, even though sera had high levels of IgE as measured by the ELISA. These findings question the significance of a positive ELISA test result and indicate that the test is not measuring functional allergen-specific IgE. (C) 2002 Elsevier Science Ltd. All rights reserved.
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